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Analysis of Pharmaceuticals with UltraSep ES MLD

簡(jiǎn)要描述:MLD packings (MLD = monolayer dense) are very stable solide supports, known in the literature as “mixed self-assembled monolayers (SAM)". They can be used both for basic and acidic compounds in a re

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Analysis of Pharmaceuticals with UltraSep ES MLD

 

UltraSep ES MLD

  

MLD packings (MLD = monolayer dense) are very stable solide supports, known in the literature as "mixed self-assembled monolayers (SAM)”. They can be used both for basic and acidic compounds in a relatively wide range of pH and with high selectivity. The buffer concentration can be held less. With organic buffers one can successfully use these columns between pH 2 – 11.

The application MLD1 demonstrates a separation on UltraSep ES MLD RP18, which was performed already on UltraSep ES PHARM RP8E with 15 mM phosphate buffer as eluent (Application PHARM3). 

With UltraSep ES MLD RP18 already £ 5 mM phosphoric acid is sufficient for delivering the same chromatogram quality (Application MLD2). Furthermore it is important that for obtaining comparable short retention times of basic pharmaceuticals one can work with 50% acetonitrile (instead of 30%). 

The application MLD3 represents a chromatogram of basic pharmaceuticals at pH 11, which was obtained after flushing the column with 6000 ml eluent, pH 11, corresponding to a continuous use of approximay 4 weeks. Remarkable is the still very good peak shape especially of perazine dimalonate.

For working with basic eluents the usage of organic buffers and the addition of organic solvents is recommended.

MLD packing are, as already mentioned, suitable for the separation of organic acids too (Application MLD4).

Using MLD packings it is possible to obtain chromatograms with good peak shapes of very different classes of compounds (Application MLD5).

   

 

Examples of Analyzed Compounds

Analyzed in Application

Go to

 

Acetic acid

MLD4

MLD1
 

Amitriptyline

MLD3

 

Chloramphenicol

MLD5

MLD2
 

Clozapin

MLD1, MLD2, MLD3

 

Diphenhydramine

MLD3

MLD3
 

Flecainide

MLD1, MLD2, MLD3

 

Formic acid

MLD4

MLD4
 

Mexiletine

MLD1, MLD2

 

Perazine dimalonate

MLD3

MLD5
 

Protriptyline

MLD1, MLD2

 

Sulfadimethoxine

MLD5

 
 

Sulfathiazole

MLD5

 

 

COLUMNS and  PRICES  ()
 Column size (mm)

200 x 3

200 x 2
150 x 3
125 x 3
150 x 2
125 x 2
UltraSep ES MLD
(RP8 or RP18 5 µm)
280.00280.00255.00255.00
 

Size (mm)

10 x 310 x 2 
Precolumn cartridges
(Price per pack = 5 pieces)
170.00170.00

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